PACT premier™ MD1-29
- A 24 well pH/PEG screen with broad range buffers to maintain consistent buffer chemistry across the pH range
- A 24 well Cation/PEG screen
- A 48 well anion/PEG screen
This PEG/ION/pH screen has been developed to systematically test the effect of pH, anions and cations, using PEG as the precipitant.
This screen has been implemented very successfully at the Netherlands Cancer Institute (NKI), and at the Oxford Protein Production Facility (OXPPF).
PACT premier (commercialized under licence which contributes back to the originator's research) - takes the popular systematic PEG/Ion approach and extends this by adding the pH parameter.
When used together with JCSG-plus™ (JCSG+) as a primary screening strategy this combination is extremely powerful and successful, being a combination of a modern sparse matrix approach and an information yielding systematic trial.
PACT premier™ is a pH, Anion, Cation crystallization screen devised to test pH within a PEG/Ion screen environment.
The kit contains 96 reagents arranged as a cation/PEG screen, an anion/PEG screen and a pH/PEG screen.
See also PACT Premier HT-96 screen
Read about a Classic Crystal Growth Screening approach to protein crystallography.Learn about a Modern Approach to Protein Crystallization Screening.
The PACT/JCSG Strategy
This systematic grid screen screen is complemented by an optimized sparse matrix screen such as JCSG-plus. Together, the two screens comprise a highly effective strategy to identify new crystallization conditions, as demonstrated by SPINE members (Newman et al, 2005. Towards rationalization of crystallization screening for small- to medium-sized laboratories: the PACT/JCSG+ strategy. Acta Cryst. D61: 1426). When combined with JCSG-plus™ this screen is the optimum starting point for growing a protein crystal (see MemGold for Membrane protein crystal growth).
The screen was developed by Janet Newman, and was tested in the laboratory of Anastassis Perrakis at the NKI as part of the SPINE programme, and is manufactured under license by Molecular Dimensions.