Last Chance Saloon!

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Last Chance Saloon!

We've all struggled with proteins and their idiosynchratic ways. Here are our suggestions for dealing with tricky macromolecules.

When you've tried everything you can think of but still have no crystals, or you need better crystals!


Here are our suggestions:

1. Make sure your protein is in the best possible starting buffer - screen 96 buffers without wasting your protein using the diaplate, or try crystallization by dialysis. Try our protein stability screens with ThermoFluor® or another assay to identify the best conditions for your protein.


2. Try the 3D Structure Screen (also known as Heavy + Light) - a little more hands on than just setting your screens and waiting, but could produce that vital crystal!
3. Try Polyvalan's Crystallophore No1. This luminescent Terbium complex is an effective nucleant and provides significant isomorphous and anomalous phasing power. So you can grow more crystals, and see tiny ones more easily with Crystallophore No1 green luminescence! In addition, once you get crystals, With the strong anomalous signal at Terbium's LIII edge (f"~30 electrons at 1.6 Å or 7.5 KeV), and significant anomalous signal even at some distance from the edge, you can solve the phase problem at the same time! Read more here.
3. Add a nucleant - read the paper here
4. Seeding - try these tips from Dr Terese Bergfors

5. There are also loads of great exercises to try in this article by Professor Naomi Chayen.

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