screens in one box: a 24 well pH/PEG screen - with broad range
buffers thus maintaining consistent buffer chemistry across the pH
range, a 24 well Cation/PEG screen and a 48 well anion/PEG screen.
This PEG/ION/pH screen has been developed to systematically test the
effect of pH, anions and cations, using PEG as the precipitant.
This screen has been implemented very successfully at the Netherlands Cancer Institute (NKI), and at the Oxford Protein Production Facility (OXPPF).
(commercialized under licence which contributes back
to the originator's research) - takes the popular systematic PEG/Ion
approach and extends this by adding the pH parameter.
When used together with JCSG -Plus
(JCSG+) as a
primary screening strategy this combination is extremely powerful and
successful, being a combination of a modern sparse matrix approach and
an information yielding systematic trial.
The screen contains 96 x 1.1 mL reagents in an SBS standard deep-well block.Keywords: PACT Screen, crystallization of proteins, Peg/Ion Screen, Newman et al, Protein structure determination.
Read about a Classic Crystal Growth Screening approach to protein crystallography.
Learn about a Modern Approach to Protein Crystallization Screening
.The PACT/JCSG Strategy
This systematic grid screen screen is complemented by an optimised sparse matrix screen such as JCSG-plus
. Together, the two screens comprise a highly effective strategy to identify new crystallisation conditions, as demonstrated by SPINE members (Newman et al
, 2005. Towards rationalization of crystallisation screening for small- to medium-sized laboratories: the PACT/JCSG+ strategy
. Acta Cryst. D61
: 1426). When combined with JCSG+ this screen is the optimium starting point for growing a protein crystal (see Memgold for Membrane protein crystal growth).The screen was developed by Janet Newman, and was tested in the laboratory of Anastassis Perrakis at the NKI as part of the SPINE programme, and is manufactured under license by Molecular Dimensions.
The screen contains 96 conditions of 1.1 mL reagents in an SBS standard deep-well block.
Keywords: PACT Screen, crystallization of proteins, Peg/Ion Screen, Newman et al, Protein structure determination.
Also referred to as the: PACT Suite and JBScreen PACT++