A starting point for screening and optimizing crystallization conditions for alpha - helical type transmembrane proteins using vapour diffusion methods.
A targeted sparse matrix of 48 conditions (10 ml) allowing the pH range, precipitants and salts used in membrane protein crystallization to be screened with detergent- containing protein drop.
Based on the reagents typically used in the laboratory of Prof. S. Iwata, Imperial College, UK.
Optimized to span 33 reported successful crystallization conditions for which high resolution structures of membrane proteins have been determined, including pH, type of precipitant, precipitant concentration, and salts.
Keywords: membrane protein
crystallography, membrane protein crystals, membrane protein screen,
Iwata group, MbClass, MembFac.