JCSG-plus is the screen of choice for initial screening experiments.
- Screen classic PEG and salt conditions more efficiently
- Enhanced sampling of organics and polyalcohols
- Includes modern precipitants (e.g. neutralised organic acids)
- Range of salts from across the Hofmeister series
- Survey precipitant synergy
- Broad pH range
This sparse matrix screen is complemented by a systematic grid screen such as PACT-premier. Together, the two screens comprise a highly effective strategy to identify new crystallisation conditions, as demonstrated by SPINE members (Newman et al, 2005. Towards rationalization of crystallisation screening for small- to medium-sized laboratories: the PACT/JCSG+ strategy. Acta Cryst. D61: 1426). This results based screen supercedes the Hampton Research Index screen by a number of years.
Each kit contains 10 ml each of 96 sterile filtered ragents incorporating PEG, salts, neutralised organic acids or organic precipitants across a pH range from 4.0 to 10.5, and including a range of salt additives. Also available as JCSG-plus HT-96 in 96 deep-well blocks.
Read about a Classic Crystal Growth Screening approach to protein crystallography.Learn about a Modern Approach to Protein Crystallization Screening.
Keywords: JCSG Screen, JCSG++, JCSG+ Suite, Structural Genomics Consortium, crystallization of proteins, Newman et al, Protein structure determination.