CryoSol™ MD1-90

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CryoSol™ is an extended set of multicomponent solutions intended for crystal cryoprotection and soaking of hydrophobic ligands into crystals.

  • Use in co-crystallization screening of protein-ligand complexes.
  • Preparation of ligand-loaded cryosolutions for soaking experiments.
  • Ideal for the solubilization of hydrophobic ligands.
  • Protein friendly mixes of DMSO/dioxane/ethylene glycol should improve ligand solubilization.
  • Contains 2,3-butanediol- compatible with enzymatic activity.
  • Mixed solutions can be used as an additive screen (up to 5% in precipitant).
  • Compliments CryoProtX.
MD1-90 is presented as 33 x 1.5 mL microtubes (18 x multi-component cryoprotectant solutions (CM1-18), 6 x solubilisation solutions (SM1-6) and 9 x stock solutions).

Ciccone L., Vera L., Tepshi L., Rosalia L., Rossello A. & Stura E.A.(2015) Multicomponent mixtures for cryoprotection and ligand solubilization. Biotechnol. Reports (7) 120-127

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