JCSG-plus Green Screen MD1-56


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JCSG-plus™ is the screen of choice for initial screening experiments.

The most complete sparse matrix screen available today
  • Screen classic PEG and salt conditions more efficiently
  • Enhanced sampling of organics and polyalcohols
  • Includes modern precipitants (e.g. neutralised organic acids)
  • Range of salts from across the Hofmeister series
  • Survey precipitant synergy
  • Broad pH range

This sparse matrix screen is complemented by a systematic grid screen such as PACT-premier. Together, the two screens comprise a highly effective strategy to identify new crystallization conditions, as demonstrated by SPINE members (Newman et al, 2005. Towards rationalization of crystallization screening for small- to medium-sized laboratories: the PACT/JCSG+ strategy. Acta Cryst. D61: 1426). This results based screen supersedes the Hampton Research Index screen by a number of years.

Each kit contains 10 ml each of 96 sterile filtered reagents incorporating PEG, salts, neutralised organic acids or organic precipitants across a pH range from 4.0 to 10.5, and including a range of salt additives. Also available as JCSG-plus™ HT-96 in 96 deep-well blocks.

Green Screens contain a non-covalent fluorescent dye which conveys fluorescence on most proteins when illuminated with UV light. This not only increases the signal to noise ratio (important for very small crystals), but also allows the identification of protein crystals lacking intrinsic fluorescence that would otherwise remain ambiguous.




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