RUBIC Additive Screen MD1-97
RUBIC Additive Screen- designed at the EMBL Hamburg and optimized for Differential Scanning Fluorimetry (ThermoFluor®) to determine optimum conditions for protein stability, purification and storage. Suitable for use with any protein stability assay.
Features of RUBIC Additive Screen:
- Wide-range of additives: salts, monovalent and multivalent ions, chaotropic reagents, NDSB’s, detergents, carbohydrates, carboxylic acids, amino acids, polyols, reducing agents, linkers, co-factors, polyamines and ligands.
- Use from protein purification all the way through to characterization by NMR, SAXS or X-ray crystallography.
- Great versatility- allows customisation of buffer compatible with protein of interest.
- Use as a silver bullet.
MD1-97 is presented as 96 x 0.25* mL conditions and 24 x 1.5mL 5X (500 mM) buffers (*enough for 15 experiments)
Boivin S, Kozak S, Meijers R. (2013) Optimization of protein purification and characterization using Thermofluor screens. Protein Expr Purif. 91(2):192-206.
Newman J. (2004) Novel buffer systems for macromolecular crystallization. Acta Crystallogr D Biol Crystallogr. 60:610-2.
RUBIC Additive Screen is free of preservatives. It is recommended that users prefill plates and store them at -20˚C or -80 ˚C if possible upon receipt.
ThermoFluor is a registered trademark of Johnson and Johnson.