The Protein Stability Combo Kit MD1-103

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Thermal shift assays provide information on the effect various environmental factors have on the thermal denaturation of a protein. Thermal denaturation is related to protein stability and therefore crystallizability. The most common thermal shift assay is ThermoFluor®, which measures increasing fluorescence induced by SYPRO® Orange binding to the exposed protein hydrophobic core. But there are many others depending on the requirements of your sample.

Our protein stability screens are now available in one package at a discount of more than 20% compared to buying them separately (or approximately 4 kits for the price of 3).

The Durham Screens

Developed by Emily Cardew and Ehmke Pohl at the University of Durham, these kits provide greater depth of analysis of the effect of individual buffers, pH and salts. The Durham screen is designed to work with NAMI, a GUI-based program to aid interpretation of thermal shift data.

The Durham pH Screen contains deconvolutes buffer molecule and pH effects for a borad range of molecule (28 different buffers) and pH (4-11).

The Durham Salt Screen uses more than 30 different salts, including a range of lanathanides. It allow discrimination between different anions and cations as well as revealing the effects of ionic strength.

Read more about The Durham Screens in: Grøftehauge, MK, Hajizadeh, NR, Swann, MJ and Pohl, E. Protein-ligand interactions investigated by thermal shift assays (TSA) and dual polarization interferometry(DPI). Acta Cryst D71:36-44 (2015).

The RUBIC Screens

Invented by Staphane Boivin and Rob Meijers at the Sample Perparation and Characterisation facility at EMBL Hamburg. The Rubics screen consists of two kits:

The RUBIC Buffer Screen deconstructs the buffer components to allow a rational interpretation and deconvolution of pH, buffer molecule and ionic strength effects.

The RUBIC Additive Screen analyzes the effect on stability of various common additives including ions, detergents, sugars, amino acids, imidazole, cryoprotectants and reducing agents.


The RUBIC Screens are described in:Boivin S, Kozak S, Meijers R. (2013) Optimization of protein purification and characterization using Thermofluor screens. Protein Expr Purif. 91(2):192-206.

Reference: Newman J. (2004) Novel buffer systems for macromolecular crystallization. Acta Crystallogr D Biol Crystallogr. 60:610-2.

Storage conditions:

The Durham pH Screen is presented as 96 x 0.5 mL conditions in a deep-well block. They are preservative free and it is recommended that they are stored at 4°C, however they can be used at room temperature for screen set-up.

RUBIC Buffer screen is free of preservatives. Shipping at Room Temperature. Short-term Storage at 4˚C. It is recommended that users prefill plates and store them at -20˚C.

ThermoFluor is a registered trademark of Johnson and Johnson. SYPRO is a registered trademark of Life Technologies.

 

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