Crystallophore No 1 (large vial , 0.6mg) MD2-80
This is the LARGE (0.6 mg) vial, with
enough compound to set up 10 x 96-well plates of your protein in complex
with 10 mM Crystallophore No1 (the recommended concentration for
Polyvalan Crystallophore No1 (patent pending) is a luminescent Terbium complex that is an effective nucleant and provides significant isomorphous and anomalous phasing power. Read more infomation about how Crystallophore No1 works here
Read in Vögeli et al, PNAS 115: 3380-3385 (2018) about how Crystallophore No1 was used in the crystal growth and phasing of a novel protein complex.
And its use solving the structure of Isoprenoid Lipid Ubiquinone: Chehade, MH et al, Cell Chem. Biol. online ahead of print (2019).
Crystallophore No1 acts as a nucleating agent and improves crystal quality of pre-existing hits. As shown right for T5 phage with and without Crystallophore No1 (top right).
Crystallophore No1 luminesces strongly in the visible region under UV excitation making detecting crystals in drops and loops easy. See middle right.
Terbium is a lanthanide and therefore has significant potential for use as an anomalous scatterer and in traditional isomorphous replacement. When Crystallophore No1 has been used as a nucleant, it is often found bound to the protein thus providing high quality phasing power.
READ ALL ABOUT IT
The development work leading to Crystallophore No1 has now been published in:
Engilberge, S., Riobé, F., Di Pietro, S., Lassalle, L., Coquelle, N., Arnaud, C.-A., Pitrat, D., Mulatier, J.-C., Madern, D., Breyton, C., Maury, O. and Girard, E. Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effect, phasing properties and luminescence. Chemical Science 8: 5909-5917 (2017).
(bottom right) P. horikoshii Protease 1 electron density from SAD phasing using the Terbium LIII absorption edge. Crystal grown in 10 mM and subsequently soaked in 100 mM crystallophore No1.
Each large vial ccontains 0.6 mg of the complex, enough to set up 10 96-well plates of your target protein in complex with 10 mM Crystallophore No1 (recommended concentration). Shipped at room temperature. The dry powder will keep for upto 1 year in a sealed tube at room temperature in dry conditions. Solutions of Crystallophore No1 can be kept for up to 2 months in a sealed tube. Solutions of the compound mixed with your target protein should be used immediately.