RUBIC Additive Screen SPECIAL MD1-97-SPECIAL

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SPECIAL CLEARANCE OFFER

(Product is more than 12 months old).

RUBIC Additive Screen- designed at the EMBL Hamburg and optimized for Differential Scanning Fluorimetry (ThermoFluor®) to determine optimum conditions for protein stability, purification and storage. Suitable for use with any protein stability assay.

Features of RUBIC Additive Screen:
  • Wide-range of additives: salts, monovalent and multivalent ions, chaotropic reagents, NDSB’s, detergents, carbohydrates, carboxylic acids, amino acids, polyols, reducing agents, linkers, co-factors, polyamines and ligands.
  • Use from protein purification all the way through to characterization by NMR, SAXS or X-ray crystallography.
  • Great versatility- allows customisation of buffer compatible with protein of interest.
  • Use as a silver bullet.

MD1-97 is presented as 96 x 0.25* mL conditions and 24 x 1.5mL 5X (500 mM) buffers (*enough for 15 experiments)

References:
Boivin S, Kozak S, Meijers R. (2013) Optimization of protein purification and characterization using Thermofluor screens. Protein Expr Purif. 91(2):192-206.
Newman J. (2004) Novel buffer systems for macromolecular crystallization. Acta Crystallogr D Biol Crystallogr. 60:610-2.

Storage conditions:
RUBIC Additive Screen is free of preservatives. Shipping is on ice. Product may thaw during shipping; this will in no way affect its use. It is recommended that users prefill plates and store them at -20˚C or -80 ˚C if possible upon receipt.

ThermoFluor is a registered trademark of Johnson and Johnson.

Clearance offers on crystallization screens are excess stock and have been in stock for a year to 18 months since their manufacture date. These screens have been stored in ideal conditions (4oC in the dark) and are sterile until opened.

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