NAPol - Non-ionic Amphipol NAP-10/100/250/500-MG
Non-Ionic Amphipol, Glycosylated; Non-Ionic Amphiphilic Homopolymer.
Molecular Formula: C7H15O4SN (C31H54O15N2)n
Formula Weight: 7000-35000g/mol; (n=10-50)
Amphipathic surfactant for maintaining solubilized membrane proteins in detergent-free solutions. First developed at the University of Avignon(1), and further characterized at the CNRS(2), each repeating unit of NAPol contains two glucose molecules and one undecyl chain. The non-ionic nature of this amphipol means that it is soluble across a wide pH range, and is compatible with multivalent cations. It is compatible with cell-free synthesis of membrane proteins, as well as NMR studies.
A number of publications have demonstrated the utility of NAPol in membrane protein structure determination(3), (4), (5). Interestingly, density of the NAPol can be seen in the Cryo-EM projection map of C. reinhardtii photosystem I supercomplex in Kubota-Kawai et al(4). In addition, the NAPol-based purification method described in Burton-Smith et al(5) yielded the largest photosynthetic supercomplex at the highest level of intact configuration reported to date.
Read more about amphipols in the August 2019 issue of the Anatrace newsletter.
Available in 10mg, 100mg, 250 mg and 500mg amounts.
References(1) Sharma, VS et al. Langmuir 28: 4625-39 (2012).
(2) Bazzacco, P et al. Biochemistry 51: 1416-30 (2012).
(3) Bausewein, T et al. Cell 170: 693-700 (2017).
(4) Kubota-Kawai, H et al. J. Biol. Chem. 294: 4304-4314 (2019).
(5) Burton-Smith, RN et al. J. Biol. Chem. epub ahead of print (2019).