A rational approach to screening initial crystallization conditions of nuclear receptors ligand binding domains.
The NR-LBD™ screen contains 48 conditions divided into two parts: 24 conditions using various PEGs as the precipitant and 24 conditions using various carboxylic acids as the precipitant.The extension contains a set of 48 unique reagents which not only allows the user to reproduce previously obtained results but also to crystallize new ligand binding domains.
Features of the Nuclear Receptor - Ligand Binding Domain™ screen:
- Nuclear receptors usually crystallize in the presence of two reagent families: polyethylene glycol and carboxylic acids.
- The pH range for crystallization is 6.0 to 9.0.
- Includes PEGs 4000, 6000 and 8000.
- Includes sodium formate, sodium citrate, sodium acetate carboxylic acids.
- Ionic strength controlled with sodium chloride and ammonium
acetate. pH range is between 6.5 and 8.5 using Bis-Tris (pH 6.5), PIPES
(pH 7.0), sodium HEPES (pH 7.5) and Tris (pH 8.0 and 8.5).
Also effective with other soluble proteins.
NR-LBD screen and the NR-LBD extension screen were developed by Denis
Zeyer, Sylvie Duclaud, Dino Moras and Jean-Paul Renaud at The Institute
of Genetics and Molecular and Cell Biology, CNRS, UMR 7104, Illkirch,
France and is manufactured under an exclusive licence from CNRS, France.
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