Crystals can be further improved by adding substrate analogues, inhibitors, ligands or other additives.
Some tips for additive screening.
Additive Screening can be useful for small/poorly diffracting crystals or too much nucleation. There are a variety of approaches that can be taken:
- Using any of our additive screens (for soluble or membrane proteins) - use your hit condition as a base.
- Look for chemical entities in common amongst your initial hits. Re-screen with one or more of the
primary screens using this entity as an additive.
- If your protein is known to bind a ligand, re-screen with one or more of the primary screens using the ligand as an additive.
- Perform a thermofluor experiment testing your protein against the additive screens. If you observe a significant stabilisation of your protein, re-screen with one or more of the primary screens using the stabilising condition as an additive.
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