PACT premier


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PACT <i>premier</i>™

PACT premier™ is a pH, Anion, Cation crystallization trial devised to test pH within a PEG/Ion environment. This is one of the most effective systematic screens available.

Building on the success of PEG/Ion screening, this PEG/ION/pH screen has been developed to systematically test the effect of pH, anions and cations, using PEG as the precipitant. This screen was originally implemented very successfully at the Netherlands Cancer Institute (NKI), and at the Oxford Protein Production Facility (OXPPF). The kit contains 96 reagents arranged as a cation/PEG screen, an anion/PEG screen and a pH/PEG screen.

Features of PACT premier™:
A modern, comprehensive PEG/ion screen.
This 96-well screen is really 3 screens:
1. 24-well pH/PEG screen.
2. 24-well cation/PEG screen.
3. 48-well anion/PEG screen.

The screen was developed by Janet Newman, and was tested in the laboratory of Anastassis Perrakis at the NKI as part of the SPINE programme, and is manufactured under license by Molecular Dimensions.

Newman et al, (2005), Acta Cryst. D61, 1426

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