The Lipidic-Sponge Phase™ Screen
SHARE
The sponge phase is an expanded mesophase where the aqueous channels are larger than in the cubic phase, thus it is able to accommodate membrane proteins with large extra- and/or intracellular domains.
It is obtained by mixing Monoolein (MO) with a buffer solution, and a crystallization agent, such as PEG or Jeffamine M-600. Salt is also added to the buffer and the pH varied, allowing a broad variety of distinct sponge phases to be formed.
Features of The Lipidic-Sponge Phase™ Screen:
- Guaranteed lipidic-sponge phases, premixed and ready-to-use!
- Closely resembles the native lipidic environment of membrane proteins.
- Suitable for use in hanging or sitting-drop vapour diffusion experiments, and in lamina.
- Easy to handle liquids.
- Easy retrieval of protein crystals.
- No lipase or cryoprotectants needed.
- Can be used with additive and crystallization screens.
- Easy to spot colourless crystals.
- Readily compatible with high-throughput crystallization approaches.
References
Annemarie B. Wöhri, Linda C. Johansson, Pia Wadsten-Hindrichsen, Weixiao Y. Wahlgren, Gerhard Fischer,
Rob Horsefield, Gergely Katona, Maria Nyblom, Fredrik Öberg, Gillian Young, Richard J. Cogdell, Niall J.
Fraser, Sven Engström, Richard Neutze. "A lipidic-sponge phase screen for membrane protein
crystallisation” Structure, Vol 16, 1003-1009 (2008).
Page 1 of 1
[1]
