The Lipidic-Sponge Phase™ Screen

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The Lipidic-Sponge Phase™ Screen

The sponge phase is an expanded mesophase where the aqueous channels are larger than in the cubic phase, thus it is able to accommodate membrane proteins with large extra- and/or intracellular domains.

It is obtained by mixing Monoolein (MO) with a buffer solution, and a crystallization agent, such as PEG or Jeffamine M-600. Salt is also added to the buffer and the pH varied, allowing a broad variety of distinct sponge phases to be formed.

Features of The Lipidic-Sponge Phase™ Screen:

  • Guaranteed lipidic-sponge phases, premixed and ready-to-use!
  • Closely resembles the native lipidic environment of membrane proteins.
  • Suitable for use in hanging or sitting-drop vapour diffusion experiments, and in lamina.
  • Easy to handle liquids.
  • Easy retrieval of protein crystals.
  • No lipase or cryoprotectants needed.
  • Can be used with additive and crystallization screens.
  • Easy to spot colourless crystals.
  • Readily compatible with high-throughput crystallization approaches.

References
Annemarie B. Wöhri, Linda C. Johansson, Pia Wadsten-Hindrichsen, Weixiao Y. Wahlgren, Gerhard Fischer,
Rob Horsefield, Gergely Katona, Maria Nyblom, Fredrik Öberg, Gillian Young, Richard J. Cogdell, Niall J.
Fraser, Sven Engström, Richard Neutze. "A lipidic-sponge phase screen for membrane protein
crystallisation” Structure, Vol 16, 1003-1009 (2008).


  • Description

    Monoolein, 9.9 MAG

  • Price

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