The Lipidic-Sponge Phase™ Screen
The sponge phase is an expanded mesophase where the aqueous channels are larger than in the cubic phase, thus it is able to accommodate membrane proteins with large extra- and/or intracellular domains.
It is obtained by mixing Monoolein (MO) with a buffer solution, and a crystallization agent, such as PEG or Jeffamine M-600. Salt is also added to the buffer and the pH varied, allowing a broad variety of distinct sponge phases to be formed.
Features of The Lipidic-Sponge Phase™ Screen:
- Guaranteed lipidic-sponge phases, premixed and ready-to-use!
- Closely resembles the native lipidic environment of membrane proteins.
- Suitable for use in hanging or sitting-drop vapour diffusion experiments, and in lamina.
- Easy to handle liquids.
- Easy retrieval of protein crystals.
- No lipase or cryoprotectants needed.
- Can be used with additive and crystallization screens.
- Easy to spot colourless crystals.
- Readily compatible with high-throughput crystallization approaches.
Annemarie B. Wöhri, Linda C. Johansson, Pia Wadsten-Hindrichsen, Weixiao Y. Wahlgren, Gerhard Fischer,
Rob Horsefield, Gergely Katona, Maria Nyblom, Fredrik Öberg, Gillian Young, Richard J. Cogdell, Niall J.
Fraser, Sven Engström, Richard Neutze. "A lipidic-sponge phase screen for membrane protein
crystallisation” Structure, Vol 16, 1003-1009 (2008).
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