MemMagic Bicelle Screen Kit
The MEMMAGIC™ Bicelle Screen kit is based on the use of bicelles as an alternative method for the crystallization of membrane proteins in a lipidic enviorment.
Membrane proteins can be readily reconstituted into bicelles and are maintained in a native-like bilayer environment, which can be manipulated with almost the same ease as detergent solubilized membrane proteins, making it compatible with standard highthroughput screening methods.
Features of the MemMagic® Bicelle Screen Kit:
- Handles like detergent
- Behaves like lipid
- Robotically compatible
- Assay Development
MemMagic® Bicelle Screen kit provides four bicelle solutions of 40%, 35%, 30%, 25% DMPC:CHAPSO (2.8:1).
Bicelles are disk-like micelles formed by the mixture of a phosphatidylcholine lipid such as Dimyristoylphosphatidylcholine (DMPC) and a detergent such as 3-[(3-cholamidopropyl) dimethylammonio]-2-hydroxy- 1-propanesulfonate (CHAPSO). The bicelle discs can be described as patches of lipid bilayers with detergent
molecules lining the apolar edges of each bilayer.
MemMagic® and MemX® are registered trademarks of MemxBiosciences LLC.
Faham, S., Ujwal, R., Abramson, J. and Bowie, J. U. (2009) "Practical Aspects of Membrane Proteins Crystallization in Bicelles” Current Topics in Membranes, Volume 63, Chapter 5, 111-127.
Faham, S., Boulting, G. L., Massey, E. A., Yohannan, S., Yang, D., & Bowie, J. U. (2005). "Crystallization of bacteriorhodopsin from bicelle formulations at room temperature”. Protein Science, 14, 836–840.
Faham, S., & Bowie, J. U. (2002). "Bicelle crystallization: A new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure”. Journal of Molecular Biology, 316, 1–6.
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