This footprint screen for the crystallization of protein complexes and other samples with heterogeneous solubility, screens the proteion precipitant solubility curve rather than setting up a randomized crystallization trial. 1-4
The screens are presented as two sets of 24 conditions at acidic, neutral and basic pH's for polyethylene glycols and salts.
Features of MacroSol™:
- Screens the protein precipitant solubility curve rather than a crystallization trial.
- Satisfies dual requirements: – Maintain protein-protein interactions. – Reduce solubility of complex.
- Test the relative protein solubility with precipitants that have been used successfully in the crystallization of many proteins.
- Once initial crystals or crystalline aggregates are obtained from the initial screening, streak seeding is recommended to determine the ranges of conditions under which crystal growth can proceed.
1. Stura E.A., Nemerow G.R., Wilson I.A (1992), Journal of Crystal Growth 122, 273-285
2. Stura E.A. (1999) Strategy 3: Reverse Screening. In "Crystallization of Proteins: Techniques, Strategies and
Tips. A laboratory manual" (Bergfors T. ed.) International University Line pp113-124.
3. Stura E.A, Satterthwait, A.C, Calvo, J.C, Kaslow, D.C, Wilson, I.A. (1994), Acta Cryst. D50, 448-455.
4. Cartron et al, Acta Cryst. (2007). F63, 37-41 [doi:10.1107/S174430910604509X]
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