ProPlex™

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ProPlex™

ProPlex™ is formulated for the crystallization of protein complexes.

Developed and tested by Sergei Radaev et al1,2 at the NIH National Institute of Allergy and Infectious Diseases.

The kit contains a 96 condition, targeted sparse matrix screen.

Features of ProPlex™:

  • Targeted sparse matrix screen.
  • Based on results of Protein Complex Crystallization Database.
  • Satisfies dual requirements: – Maintain protein-protein interactions. – Reduce solubility of complex.
  • Medium and High MW PEGs.
  • Lower PEG concentrations.
  • Fewer organic precipitants.
  • Neutralised organic acids.
  • pH range from 4.0 – 8.5.

References
1. Radaev S., Li S. and Sun P. D. (2006), Acta Cryst. D62, pp 605-612.
2. Radaev S., Li S. and Sun P. D. (2002), J. Appl. Cryst. 35, 674-676
3. Dafforn (2007) So how do you know you have a macromolecular complex? Acta Cryst. D63, 17-25.
4. Crystallisation of Nucleic Acids and Proteins, Edited by A. Ducruix and R. Giegé, The Practical Approach Series, Oxford Univ. Press, 1992.
5. Protein Crystallization Techniques Strategies & Tips, (Bergfors T. ed.) IUL 1999.


  • Description

    ProPlex™

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  • Description

    ProPlex™ HT-96

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