Read in Vögeli et al, PNAS 115: 3380-3385 (2018) about how Crystallophore No1 was used in the crystal growth and phasing of a novel protein complex.
Crystallophore No 1 acts as a nucleating agent and improves crystal quality of pre-existing hits. As shown top left for T5 phagöe with and without Crystallophore No1.
Luminesces strongly in the visible region under UV excitation making detecting crystals in drops and loops easy. See middle left.
Terbium is a lanthanide and therefore has significant potential for use as an anomalous scatterer and in traditional isomorphous replacement. When the complex has been used as a nucleant, it is often found bound to the protein thus providing high quality phasing power.
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The development work leading to Crystallophore No1 has now been published in:Engilberge, S., Riobé, F., Di Pietro, S., Lassalle, L., Coquelle, N., Arnaud, C.-A., Pitrat, D., Mulatier, J.-C., Madern, D., Breyton, C., Maury, O. and Girard, E. Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effect, phasing properties and luminescence. Chemical Science 8: 5909-5917 (2017).
(bottom left) P. horikoshii Protease 1 electron density from SAD phasing using the Terbium LIII absorption edge. Crystal grown in 10 mM and subsequently soaked in 100 mM crystallophore No1.
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