Polyvalan Crystallophore No1(patent pending) is a luminescent Terbium complex that is an effective nucleant and provides significant isomorphous and anomalous phasing power. Read more about how Crystallophore No1 works here.
BREAKING NEWS: The first novel structure solved using Crystallophore No1, Bacteriophage T5 distal tail protein (PDBID:5MF2) has just been released in the Protein DataBank. Could yours be the next? Tell us about it!
Crystallophore No 1 acts as a nucleating agent and improves crystal quality of pre-existing hits. As shown top left for T5 phage with and without Crystallophore No1.
Luminesces strongly in the visible region under UV excitation making detecting crystals in drops and loops easy. See middle left.
Terbium is a lanthanide and therefore has significant potential for use as an anomalous scatterer and in traditional isomorphous replacement. When the complex has been used as a nucleant, it is often found bound to the protein thus providing high quality phasing power.
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The development work leading to Crystallophore No1 has now been published in:Engilberge, S., Riobé, F., Di Pietro, S., Lassalle, L., Coquelle, N., Arnaud, C.-A., Pitrat, D., Mulatier, J.-C., Madern, D., Breyton, C., Maury, O. and Girard, E. Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effect, phasing properties and luminescence. Chemical Science 8: 5909-5917 (2017).
(bottom left) P. horikoshii Protease 1 electron density from SAD phasing using the Terbium LIII absorption edge. Crystal grown in 10 mM and subsequently soaked in 100 mM crystallophore No1.
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