Cryo-EM Grid-boxes and Grid-box Storage


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Cryo-EM Grid-boxes and Grid-box Storage

Efficient, Traceable Storage and Shipping for Cryo-EM Grids

Our new grid-boxes from Swissci has been developed in collaboration with Dr Jan Löwe at the MRC Laboratory of Molecular Biology in Cambridge. Backed by years of experience in Cryo-EM, it includes many improvements on current models including antistatic polymer to prevent charge build-up, no screw-design that turns with laboratory tweezers and cost-effective pricing.

Our storage system allows you to easily track your grid-boxes for storage and shipping. Each grid-box is uniquely located by chamber number and puck identifier. Researchers and microscope users can quickly and reliably identify samples of interest – even where the grid-maker and electron microscope user are in different countries – or continents!

The pucks are 67 mm in diameter and compatible with canisters of this width. The shelved storage is compatible with Taylor-Wharton HC35 dewars. Pucks can be shipped in CX100 dewars using UniPuck shipping canisters. They are fully compatible with Uni-puck canisters, accessories and tools.

Each puck carries a unique serial number, which can be customised on request and each gridbox chamber in the puck is individually numbered. A semi-transparent rotating perspex lid allows access to a single grid-box at a time, while holding the remainder securely in the puck. There is no access to any chamber when the lid is oriented ready for placing in storage or shipping canisters.

On the left are the pucks in a Uni-puck 7-shelved storage canister (1 in picture; MD7-611), with a locking pin (2 in picture) that secures all the pucks in the canister both while in the dewar and during transfer. The pucks may be easily manipulated with our angled cryo-tongs (3 in picture; MD7-604). Cryo-EM puck-specific shelved storage and shipping canisters will be available shortly with more shelves (due to decreased depth of the cryo-EM puck compared to a Uni-puck).

These canisters are based on an idea first suggested by Dr Matthew Iadanza of the University of Leeds and we thank Dr Raffaella Carzaniga of the Crick Institute and Dr Natasha Lukoyanova of Birkbeck College, London for their advice.


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