Choosing LCP PlatesBackground
Laminex™ is a versatile system for crystallizing proteins between two planar surfaces particularly useful for in meso phase methods normally referred to as lipidic cubic phase or sponge phase which are lipid bilayer 3 dimensional structures capable of supporting membrane proteins in a manner similar to a natural biological membrane.
Laminex offers considerable advantages for viewing and imaging such crystal growth experiments since the viscous ”drop” experiment is sandwiched between planar surfaces. The flat optical path then creates no aberrations even when using lipidic cubic phases.
Crystals grown using MemMeso™ in Laminex plates
The system is also available to either fit onto an SBS frame or frameless, in which case the lower plate is itself SBS size. The choice of size will depend on the imaging system to be used. Imaging hotel systems that use a gripper arm that holds the sides of sbs plates will need to use the Laminex Frame and standard size Laminex plates. Imaging hotel system where the gripper arm supports the sandwich and is higher capacity for frameless systems require the sbs size Laminex plates (Laminex-HT).
The user can choose materials based on their personal preferences and priorities to make up a complete sandwich kit.
Glass systems are the most popular, however limit the possibility of using UV fluorescence for protein crystal identification since glass absorbs UV light. Crystals grown in meso phases are extremely difficult to see and so UV transparent plastics may be chosen instead. If using a glass cover sheet crystals can be harvested by scoring with a tungsten scriber a double square around the well and pressing on one corner to raise a window cover to access the ”drop” - detailed instructions here.
Plastic systems are more useful for imaging with UV fluorescence. In additon to the two base plates with spacers (100 & 200 µm) we offer two covers, the more popular 100µm film cover is easier to use when harvesting crystals as it can be cut with a scalpel blade. This film cover has a limited permeability to water vapour so may not be preferred for long-term crystal growth experiments - In such cases a 200µm film is recommended although not as easy to cut.
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