- New, improved version of the original revolutionary screen
- Based on alternative precipitants
- Now with PPGBAs to increase polymer diversity
- Ideal for macromolecular complexes due to the narrow range of pH and salt, centred on physiological values
- Each condition contains at least one alternative polymeric precipitant
- Designed to enhance PEG- and salt-based screening
The perfect screen for difficult to crystallize targets, MIDAS™ was a revolutionary screen that broke away from traditional sparse matrix screening using PEG or salt as a precipitant. MIDASplus™ is an evolution of that original product: still containing many of the original components but with 21 conditions altered to accommodate a new range of alternate precipitants.
MIDASplus™ contains polypropylene glycol bis-aminopropylether (PPGBAs) to increase polymer diversity. The screen still includes Jeffamine® polyetheramines, pentaerythritol propoxylate and pentareythritol ethoxylate, polyvinyl pyrrolidone, polypropylene glycol, polyvinyl alcohol and polyacrylate polymers from the original MIDAS™. MIDASplus™ is the only screen which utilises many of these compounds, which are only sporadically utilized in crystallization trials.
MIDASplus™, like MIDAS™ before it, was devised and tested in the laboratory of Dr Clemens Grimm and Prof Dr Utz Fischer at Würzburg University, Germany. The development of MIDAS™ is described in Grimm, C, Chari, A, Reuter, K and Fischer, U, Acta Cryst D66: 685-697. Both screens are manufactured and distributed by Molecular Dimensions under an exclusive licence.
PDB identifiers for structures of crytals grown from conditions included in MIDASplus™
Bleuler-Martinez, S, Stutz, K, Sieber, R, Collot, M, Mallet, J-M, Hengartner, M, Schubert, M, Varrot, A and Künzler, M (2017). Glycobiology In press.
Leupold, S, Büsing, P, Mas, PJ, Hart, DJ and Scrima, A (2017). J Struct Biol In press.
Pomowski, A, Uson, I, Nowakowska, ZM, Veillard, F, Sztukowska, MN, Guevara, T, Goulas, T, Mizgalska, D, Nowak, ML, Potempa, BA, Huntington, JA, Potempa, J and Gomis-Rüth, FX (2017). J Biol Chem In press.
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