MemChannel and MemTrans

The first screens to target specific functional and structural subsets of membrane proteins.

FeaturesTwo new screens that uniquely target specific functional groups of membrane proteins. MemChannel targets ion channels, while MemTrans is specific for transporter proteins.

Our popular MemGold™1 and MemGold2™1,2 screens have enabled the crystallisation of many membrane protein structures. The developer of those screens, Prof Simon Newstead of Oxford University, has continued to maintain his hand-curated database3 of α-helical membrane protein structures and their crystallisation conditions.

Today, this dataset3 is large enough that it is possible not just to identify the most common crystallisation conditions for membrane proteins in general, but also to analyse the differences in growth conditions between functionally-related sub-groups in a statistically robust manner. This has resulted in the development of a new generation of screens from Prof Newstead that target important groups of α-helical membrane proteins.

Dr Joanne Parker, one of the developers of the new screens commented, "In the Newstead group we typically use ... MemGold and MemGold2 and now include both MemTrans and MemChannel as standard to try and increase the chance of success. If a group is limited in the number of screens it can purchase then I would start with [MemGold and MemGold2] and then choose either MemTrans or MemChannel depending on the protein type of interest."

MemChannel™

The best screen for your ion channel protein:
  • Based on approximately 150 ion channel structures in the database.
  • Contains the most commonly occurring ion channel crystallisation conditions to maximise your chances of success.
  • Specifically targeted at this large group of pharmaceutically important structures.
  • Continually updated database3 ensures most recent hit conditions are present.
  • Hand-curated to remove false positives from unrelated structures – only ion channel conditions contribute to the formulation.
Ion channels represent around 30% of all known membrane protein structures. MemChannel gives you the best chance of crystallising your channel of interest. According to Dr Joanne Parker, ion channels, which often possess large soluble domains, require higher salt concentrations and either a lower percentage of small or medium PEG or a low percentage of a large PEG. In addition, channels have a trimodel pH profile with peaks at 4.5, 6.5 and 8. MemChannel's conditions are based on these observations and cover all the most common crystallization conditions for ion channels.

MemTrans

The only screen specifically for transporter proteins:

  • The most common crystallisation conditions identified from ~150 transporter protein structures.
  • Contains the most commonly occurring ion channel crystallisation conditions to maximise your chances of success.
  • Specifically targeted at this large group of pharmaceutically important structures.
  • Continually updated database ensures most recent hit conditions are present.
  • Hand-curated database3 to remove false positives from unrelated structures – only ion channel conditions contribute to the formulation.
Approximately 30% of all known membrane protein structures are transporter proteins. Transporters, which do not generally have large soluble domains, tend to crystallize in higher percentages of small Molecular Weight PEGS, with a skewed normal distribution of pH having a maxima of 7-7.5, according to the analysis by Prof Newstead and Dr Parker. MemTrans contains conditions based on these observations, and brings together all the most common transporter protein crystallization conditions in one place. Crystallising your transporter protein has never been so easy.

References



*MemChannel and MemTrans were developed by Prof. Simon Newstead and Dr Joanne Parker of Oxford University and exclusively licensed to Molecular Dimensions Ltd by Oxford University Innovation.

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